期刊
MICROCHIMICA ACTA
卷 186, 期 6, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3464-z
关键词
Gold nanoparticles; Platinum; enzyme mimic; Nanoflower; Lateral flow immunoassay
资金
- National Institute of Health, Centers of Biomedical Research Excellence (NIH, COBRE) [P20 GM109024]
- National Natural Science Foundation of China [31700735]
- National Natural Science Foundation of Anhui Province [1908085 MB54, 1808085QH264]
The authors describe the preparation of gold-platinum nanoflower (AuPt NFs) and show that they can be simultaneously used as a label and as an enzyme mimic in lateral flow immunoassays (LFIs). The AuPt NFs were prepared by growing Pt nanowires on the surface of gold nanoparticle. The assay involves the capture of target proteins (here: rabbit IgG as a model analyte) by the immobilized capture antibody, and by using AuPt NF-labeled secondary antibody. The AuPt NFs are thus captured by the test zone and produce a characteristic black band for visual detection of the antigen (IgG). The coloration of the test line can be further enhanced by addition of the chromogenic substrate 3-amino-9-ethyl-carbazole which is catalytically oxidized by the captured Pt nanowires on the AuPt NF and produce a red coloration. Quantitative results were obtained by reading the test line intensities with a portable strip reader. The LFI has a 5pgmL-1 detection limit for IgG under optimized experimental conditions. This is 100 times lower than that of the conventional AuNP-based LFI. Conceivably, this assay has a wide scope in that it may be applied to numerous other targets for which appropriate antibodies are available.
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