期刊
MICROBIOLOGY AND IMMUNOLOGY
卷 63, 期 5, 页码 172-178出版社
WILEY
DOI: 10.1111/1348-0421.12683
关键词
cytomegalovirus; IgG avidity index; IgM
资金
- Ministry of Health, Labour and Welfare [H25-jisedai-003, H20-kodomo-007]
- Takeda Science Foundation
- Intelligent Cosmos Academic Fundation
- Kurozumi Medical Foundation
- Ministry of Education, Culture, Sports, Science and Technology [16K10097, 25870580, 22791034]
- Grants-in-Aid for Scientific Research [16K10097, 22791034, 25870580] Funding Source: KAKEN
Primary cytomegalovirus (CMV) infection during pregnancy often results in congenital CMV infection with severe clinical complications. IgM antibodies are one of the indices of primary infection. The IgG avidity index (AI) is also known to remain low for 3 months after primary infection. Here, we evaluated and compared the performance of CMV IgM and IgG avidity assays. Because sensitivity and specificity reportedly differ between CMV IgM kits, CMV IgM detection was compared between the two commercially available ELISA kits that are most commonly used in Japan. Sera for CMV IgM were first screened using a traditional indirect ELISA kit. Selected samples were then tested for CMV IgM and CMV AI using a CMV IgM-capture ELISA kit and a CMV IgG avidity assay, respectively. The rate of concordance between the IgM kits was 89% (42/47), indicating the absence of any significant difference. Most of the CMV IgM-positive plasma samples showed high CMV IgG AI; however, 18 commercially available plasma samples with low CMV IgG AI were all CMV IgM-positive. One plausible explanation for this discrepancy is that the duration of low IgG AI is shorter than that of IgM positivity. Alternatively, CMV IgM tests may generate pseudo-positive readouts in cases of congenital infection. Nevertheless, our study confirms that CMV IgG AI can be a reliable indicator of CMV primary infection.
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