期刊
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 98, 期 -, 页码 969-981出版社
ELSEVIER
DOI: 10.1016/j.msec.2019.01.055
关键词
Silk fibroin; Poly(ethylene oxide) membrane; Periodontal regeneration; Electrospinning; Ultrasound sonication
资金
- FCT - Fundacao para a Ciencia e a Tecnologia [UID/Multi/50016/2013]
- Program FCT [IF/00411/2013]
- project SERICAMED [IF/00411/2013/CP1167]
- Norte2020
- Fundo Europeu de Desenvolvimento Regional in the frame of Programa Interreg V A Espanha - Portugal (POCTEP) 2014-2020 [0245_IBEROS_1_E]
- Fundação para a Ciência e a Tecnologia [UID/Multi/50016/2013] Funding Source: FCT
In this study, silk fibroin (SF)/poly(ethylene oxide) (PEO) membranes were designed and fabricated by combining ultrasound sonication prior to electrospinning (0 to 20 min) as a strategy to physically control the rheological properties of solutions (10 to 30% w/v PEO) and to improve the spinnability of the system. PEO has proved to be essential as a co-spinning agent to assure good membrane reproducibility and enough flexibility for clinical manipulation. The rheological tests indicated that sonication greatly increased the viscosity of SF/PEO solutions and further enhanced the quality of the produced electrospun fibers with consequent improved mechanical properties in dry and wet conditions. By tuning the viscosity of the solutions using a simple sonication step prior to electrospinning, it was possible to induce water stability in the as-electrospun matrix, as demonstrated by infra-red spectroscopy. This reduced complexity in the process since it was not necessary to concentrate silk prior to electrospinning while avoiding the use of toxic solvents to perform a post-processing stabilization treatment which usually causes dimensional changes to the SF materials. Sonication pre-treatment allowed for minimizing the amount of synthetic polymer used to achieve the desirable mechanical properties (with the modulus ranging between 90 and 170 MPa), while avoiding a further water stabilization treatment. It also had a positive impact in the in vitro cell behavior of human primary periodontal ligament cells (hPDLs), resulting in a marked increase in cell proliferation. The present developed work constitutes a step forward towards simplicity and a better fabrication control of viable electrospun SF based membranes for periodontal regeneration.
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