期刊
MARINE DRUGS
卷 17, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/md17040213
关键词
guanidine alkaloids; 6-epi-monanchorin; HRESI MS; H-1 NMR spectra; marine bacteria; Vibrio sp; polychaete; Chaetopterus variopedatus; 16S rRNA gene analysis; phylogenetic reconstruction
资金
- RSF (Russian Science Foundation) [17-14-01065]
Twenty-three bacterial strains were isolated from the secreted mucus trapping net of the marine polychaete Chaetopterus variopedatus (phylum Annelida) and twenty strains were identified using 16S rRNA gene analysis. Strain CB1-14 was recognized as a new species of the genus Vibrio using the eight-gene multilocus sequence analysis (MLSA) and genome sequences of nineteen type Vibrio strains. This Vibrio sp. was cultured, and 6-epi-monanchorin (2), previously isolated from the polychaete and two sponge species, was found in the cells and culture broth. The presence of the 6-epi-monanchorin was confirmed by its isolation followed by H-1 NMR and HRESIMS analysis. These results showed the microbial origin of the bicyclic guanidine alkaloid 2 in C. variopedatus.
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