期刊
MARINE DRUGS
卷 17, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/md17040207
关键词
Aspergillus; naphthopyrones; cytotoxicity; endophytic fungus; Leathesia nana
资金
- National Natural Science Foundation of China [81803375]
- National Postdoctoral Program for Innovative Talents [BX201700247]
- China Postdoctoral Science Foundation [2018M630804]
- Natural Science Foundation of Shandong Province [ZR2016DQ07]
- Shandong Provincial Natural Science Foundation for Distinguished Young Scholars [JQ201722]
A study on the secondary metabolites of Aspergillus sp. XNM-4, which was derived from marine algae Leathesia nana (Chordariaceae), led to the identification of one previously undescribed (1) and seventeen known compounds (2-18). Their planar structures were established by extensive spectroscopic analyses, while the stereochemical assignments were defined by electronic circular dichroism (ECD) calculations. The biological activities of the compounds were assessed on five human cancer cell lines (PANC-1, A549, MDA-MB-231, Caco-2, and SK-OV-3), and one human normal cell line (HL-7702) using an MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide] assay. Among them, the dimeric naphthopyrones 7, 10 and 12 exhibited potent cytotoxicity. Further mechanism studies showed that 12 induced apoptosis, arrested the cell cycle at the G0/G1 phase in the PANC-1 cells, caused morphological changes and generated ROS; and it induces PANC-1 cells apoptosis via ROS-mediated PI3K/Akt signaling pathway.
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