期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 103, 期 -, 页码 228-237出版社
ELSEVIER
DOI: 10.1016/j.lwt.2019.01.018
关键词
Microencapsulation; Whey protein/chitosan coacervates; Wastes valorization; Agaricus bisporus L.; Ergosterol; Response surface methodology
资金
- Foundation for Science and Technology (FCT, Portugal)
- FEDER [NORTE-01-0145-FEDER-000006]
- European Structural and Investment Funds (FEEI) through the Regional Operational Program North 2020 [Norte-01-0247-FEDER-024479, SFRH/BPD/101413/2014, SFRH/BPD/107855/2015]
- Xunta de Galicia [POCI-01-0145-FEDER-006984]
In the present work microencapsulation was applied to an ergosterol enriched extract (EEE) from Agaricus bisporus L. and pure ergosterol (PE) using a complex coacervation process. The process was optimized following two steps: (i) using response surface methodology to optimize the conditions of temperature (T, 55 to 95 degrees C), protein-to-chitosan ratio (P/C, 0.5 to 10.5), maximizing yield (Y-1, %, w/w), efficiency (Y-2, %, w/w) and load (Y-3, %, w/w); and (ii) using the dose-response methodology to optimize the ergosterol content (E) at the optimal conditions achieved in (i). The global optimal conditions for both cases were the same (T of 55 degrees C and a P/C of 0.5), but with maximized responses of Y-1, = 75.02%; Y-2 = 100.00%, Y-3 = 12.78%, and Y-1 = 19.19%; Y-2 = 100.00%, Y-3 = 15.87%, respectively. The effect of increasing ergosterol content led to the reduction of the microencapsulation yield, but with an increase in the ergosterol load and maintenance of the microencapsulation efficiency.
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