期刊
JOURNAL OF SEPARATION SCIENCE
卷 42, 期 13, 页码 2231-2238出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201900040
关键词
ballpoint connector; high-performance liquid chromatography; liquid-phase microextraction; rhubarb; Traditional Chinese medicine
资金
- Fund for Shanxi Key Subjects construction
- Young Scientists Fund of the National Natural Science Foundation of China [81302734]
- Nature Science Foundation of Shanxi Province [201701D121145]
- Undergraduate Innovation and Entrepreneurship Training Program of Shanxi Province [20160706]
This study proposed a new ballpoint connector-protected salt-oil-salt liquid phase microextraction for extraction and enrichment of trace rhein and chrysophanol in rhubarb prior to determination of the analytes by high performance liquid chromatography. In this study, a handy ballpoint connector (between ballpoint tip and ink chamber) was used as extraction device, in which its cavity was filled with n-octanol, and the bare n-octanol in its two opening ends was covered with a thin layer of sodium chloride film. The design subtly assembled salt film onto ballpoint connector for extraction and enrichment, which greatly improved the enrichment factors of the target analytes. Moreover, the novel procedure and its extraction mechanism were described and analyzed, and several crucial parameters reflecting the extraction effect were investigated and optimized. Under optimum conditions, high enrichment factors (247 and 127), good linearities with r >= 0.9998, limits of detection (0.6-1.1 ng/mL), relative standard deviations of intra-and interday (2.2-8.8% and 4.3-8.9%), and average recoveries (97.6-98.1%), were obtained, respectively. The proposed method can not only eliminate the negative effects from viscosity and ion strength at high salt concentration of sample phase, but also make salting-out effect be focused on small area so as to maximize the extraction effect.
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