期刊
JOURNAL OF SEPARATION SCIENCE
卷 42, 期 13, 页码 2272-2279出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201900175
关键词
aptamers; ochratoxin A; monoliths; specific recognition; triazines
资金
- Scientific Research and Development Project of Fujian Province [2018YZ0002-1, 2017Y0056]
- Education and Scientific Research Project for Young Teachers of Fujian Province [JAT170105]
Developing a high-performance modification protocol is critical for efficiently fabricating affinity monolith. Herein, by using 2,4,6-trichloro-1,3,5-triazine as the linker, a simple triazine-bridged approach was proposed for efficiently fabricating aptamer-grafted polyhedral oligomeric silsesquioxane-polyethyleneimine affinity monolith with high specificity toward ochratoxin A. Experimental parameters, column characteristics and specificity performances of the resultant affinity monolith were investigated in detail. Under the optimal conditions, 2,4,6-trichloro-1,3,5-triazine was rapidly grafted on the polyamine matrix, and effectively applied to the subsequent bridge linkage of aptamers. It was simple and effective, which resulted in a significant decrease of modification time, excellent properties including the high coverage density of aptamer up to 1799 pmol/mu L and sensitive detection of ochratoxin A as low as 10 pg/mL in beer samples. This protocol provides a facile approach for fabricating aptamer-grafted polyamine affinity monoliths with highly selective discrimination performance.
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