期刊
JOURNAL OF PROTEOME RESEARCH
卷 18, 期 5, 页码 2331-2336出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00190
关键词
metabolomics; siderophore; pathogenic E. coli; drug target
资金
- National Natural Science Foundation of China [81274175, 31670031]
- National Key R&D Program of China [2017YFC1308600]
- Shanghai Jiao Tong University [WF220441502]
To date, yersiniabactin remains the only identified siderophore encoded by the high pathogenicity island (HPI) in uropathogenic Escherichia coli (UPEC). In the present study, we aim to discover and identify new siderophores in the HPI-dependent biosynthetic pathway using a combinational strategy of metabolomics and genetics. A global metabolome assay of wild-type UTI89, UTI89 Delta ybtS, and UTI89 Delta ybtS with the substrate addition of salicylic acid found numerous unknown metabolite features that were encoded by the HPI with an obvious substrate dependency on salicylic acid. One metabolite feature with m/z 307.0206 was shown to have a similar phenotype as yersiniabactin. Furthermore, isotope mass spectrum calculations and MS/MS annotations were combined to identify this metabolite as HPTzTn-COOH. HPTzTn-COOH was verified as a new siderophore in this study, and it was observed to have a robust capacity to chelate different metals, including Al3+, Ni2+, and Ca2+, in addition to binding Fe3+. Our data revealed that HPTzTn-COOH has a stronger diagnostic ability over the more conventionally used yersiniabactin, as characterized by its high production throughout UPEC strains harboring HPI. Altogether, our discoveries revise the siderophore family, and HPTzTn-COOH can be classified as an additional key siderophore along with yersiniabactin.
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