期刊
JOURNAL OF PHYSIOLOGICAL SCIENCES
卷 69, 期 4, 页码 635-642出版社
SPRINGER JAPAN KK
DOI: 10.1007/s12576-019-00681-w
关键词
SMCT1; PDZRN3; Monocarboxylate; Transporter; PDZ protein
类别
资金
- Japan Society for the Promotion of Science (KAKENHI) [15590233, 18590900, 21390073, 26461258, 18K08200]
- Strategic Research Foundation [S1412001]
- Nakatomi Foundation
- Gout Research Foundation of Japan
- Takeda Science Foundation
- Salt Science Research Foundation [0524, 0721]
- Shimabara Foundation
- Grants-in-Aid for Scientific Research [18K08200, 26461258, 21390073, 18590900, 15590233] Funding Source: KAKEN
Sodium-coupled monocarboxylate transporter SMCT1 (SLC5A8) mediates monocarboxylate transport in the proximal tubule of the kidney. We have identified PDZK1 and PDZ domain-containing RING finger 3 (PDZRN3) as potent binding partners of SMCT1, which has a PDZ motif (Thr-Arg-Leu), by yeast two-hybrid screening and revealed that PDZK1 enhances the transport activity of SMCT1. In this study, we aimed to characterize the interaction between SMCT1 and PDZRN3 as well as to examine how PDZRN3 regulates SMCT1 function. An interaction between SMCT1 and PDZRN3 through the PDZ motif was observed in a co-immunoprecipitation assay and yeast two-hybrid assay. A transport assay showed that PDZRN3 abolished the enhancing effect of PDZK1 on nicotinate uptake via SMCT1. Our results suggest that SMCT1 interacts with PDZRN3 and that PDZRN3 may regulate SMCT1 function by interfering with the interaction between SMCT1 and PDZK1.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据