4.6 Article

Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications

期刊

出版社

IOP PUBLISHING LTD
DOI: 10.1088/1361-6463/ab16b4

关键词

temporal focusing; multiphoton microscopy; quantum dots; fluorescence microscopy; neurophotonics

资金

  1. Wellcome Trust [093831/B/10/Z]
  2. NIH (Laser Biomedical Research Center) [9-P41-EB015871-26A1]
  3. European Molecular Biology Organization (EMBO) long-term fellowship
  4. NIH [5-U54-CA151884, P01-CA080124, R01-CA126642, R01-CA096915]
  5. NCI/Federal Share Proton Beam Program Income
  6. ARO through the Institute for Soldier Nanotechnologies [W911NF-13-D-0001]
  7. Department of Defense [DoD W81XWH-10-1-0016]
  8. NSF [ECCS-1449291]
  9. National Institute of Health [5R01NS051320, 4R44EB012415, 1R01HL121386-01A1]
  10. National Science Foundation [CBET-0939511]
  11. Hamamatsu Corporation
  12. Singapore-Massachusetts Institute of Technology Alliance for Research and Technology (SMART) Center, BioSystems and Micromechanics (BioSyM)
  13. Wellcome Trust [093831/B/10/Z] Funding Source: Wellcome Trust

向作者/读者索取更多资源

The first ever demonstration of temporal focusing with short wave infrared (SWIR) excitation and emission is demonstrated, achieving a penetration depth of 500 mu m in brain tissue. This is substantially deeper than the highest previously-reported values for temporal focusing imaging in brain tissue, and demonstrates the value of these optimized wavelengths for neurobiological applications.

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