期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
卷 376, 期 -, 页码 63-72出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2019.02.023
关键词
Dunaliella salina; A-NPCDs; In-vitro cellular imaging; Hg (II); Cr (VI) detection
资金
- UGC-UPE II
- [22/12/2013(ii) EU-V]
- [09/013/(0574)/2015-EMR-I]
- [09/013(0597)/2015-EMR-I]
- [R/DEV./D./DST-PURSE/5050/20244]
The impact of hazardous chemicals and toxic metal ions in the environment played havoc to the ecosystem. In the present work green chemistry approach was applied for one-step hydrothermal synthesis of nitrogen, phosphorus dual doped carbon dots, utilizing green precursor i.e, biomass of halophilic microalgae Dunaliella salina (algal derived nitrogen phosphorous carbon dots i.e A-NPCDs). Synthesized A-NPCDs were characterized through TEM, FT-IR, P-XRD, DLS and XPS. It showed appreciable optical properties with significant fluorescence quantum yield (8%)and exhibited blue color under UV - light (lambda(ex) = 365 nm). A-NPCDs acted as fluorescent turn off sensor for toxic metal ion such as Hg (II) and Cr (VI) with good selectivity and sensitivity. Interestingly, ANPCDs detected Cr (VI) up to 0.018 mu M which was below the permissible level of Cr (VI) in drinking water. Such sensing resulted because of combination of inner filter effect and dynamic quenching mechanism. Moreover, it also showed good selectivity (0.018 mu M) for Hg (II) via dynamic quenching mechanism. MTT assay of A-NPCDs on HEK-293 cell line showed biocompatibility with negligible cytotoxicity. Therefore, these were successfully employed for live cell imaging and intracellular detection of Hg (II) and Cr (VI) in HEK-293 cell line. Thus, green synthesized A-NPCDs may be a good alternative for chemically derived CDs in intracellular detection of Hg (II) and Cr (VI) of a complex biological environment.
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