期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 168, 期 -, 页码 94-101出版社
ELSEVIER
DOI: 10.1016/j.jpba.2019.01.051
关键词
Deoxynivalenol; Mimotope; Phage displayed peptide; Electrochemical immunosensor
资金
- National Key R&D Program of China [2018YFC1602203]
- National Natural Science Funds [NSFC-31671924]
- Jiangxi Province Science Fund for Distinguished Young Scholar [20171BCB23023]
- Natural Science Foundation of Jiangxi, China [20181BAB204019]
- Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201825]
Immunoassays play an important role in the monitoring and detection of mycotoxins in food. However, mycotoxin conjugates in the immunoassay system synthesized with toxic mycotoxin as raw material has seriously harmed the environment and human health during and after the performance. In this work, using mycotoxin deoxynivalenol (DON) as a model happen, a selected DON mimotope (phage-displayed peptide) that specifically binds to anti-DON antibody was served as a mimetic competing antigen in electrochemical immunosensor for ultrasensitive and green detection of DON. Under the optimized conditions, the mimotope peptide-based electrochemical immunosensor showed a dynamic range of 0.1-10,000 pg/ml and the limit detection (LOD) of 0.07 pg/ml. In addition, the accuracy and reproducibility of the established electrochemical immunosensor were evaluated by testing cross-reactivity and spiking DON negative samples. The result showed that mimotope peptide-based immunosensor has negligible cross-reactivity with other mycotoxins, and the average recovery of corn and wheat sample was 90.4%-118% and 101.3%-111%, respectively. Furthermore, the novel concept might provide potential applications to a general method for the immunosensor of various toxic small molecules. (C) 2019 Elsevier B.V. All rights reserved.
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