期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 68, 期 6, 页码 961-972出版社
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.001000
关键词
antimicrobial peptides; antibiotics; polymethylmethacrylate bone cement; biofilm; implant-related infections
类别
资金
- Ministry of Health of the Czech Republic [16-27726A]
- Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences [RVO 61388963]
- Motol University Hospital [9777, 6010]
Purpose. Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated with the formation of bacterial biofilms. In the context of emerging bacterial resistance to current antibiotics, we examined the efficacy of short antimicrobial peptide-loaded bone cement in inhibiting bacterial adhesion and consequent biofilm formation on its surface. Methodology. The ability of alpha-helical antimicrobial peptides composed of 12 amino acid residues to prevent bacterial biofilm [methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli] formation on the surface of model implants made from polymethylmethacrylate-based bone cement was evaluated by colony-forming unit (c.f.u.) counting of bacteria released by sonication from the biofilms formed on their surfaces. The biofilms on model implant surfaces were also visualized by light microscopy after staining with tetrazolium dye (MTT) and by scanning electron microscopy. Results. When incorporated in the implants, these peptides caused a mean reduction in the number of bacterial cells attached to implants' surfaces (by five orders of magnitude), and 88 % of these implants showed no bacterial adhesion after being exposed to growth media containing various bacteria. Conclusion. The results showed that the antibiofilm activity of these peptides was comparable to that of the antibiotics, but the peptides exhibited broader specificity than the antibiotics. Given the rapid development of antibiotic resistance, antimicrobial peptides show promise as a substitute for antibiotics for loading into bone cements.
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