期刊
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷 42, 期 11-12, 页码 351-357出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2019.1598429
关键词
Biphenyl column; mobile phase; pentafluorophenylpropyl column; polyphenols; UFLC analysis
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) vide research Project Instituto Nacional de Ciencia e Tecnologia de Frutos Tropicais [57573781/2008-7]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brazil under Programa Nacional de Pos-doutorado [PNPD/CAPES-086/2013, 001]
The development of efficient analytical methods for the identification and quantification of polyphenols in natural products is needed. The present study was aimed to optimize the polyphenols by UFLC using two different Ascentis express columns. Chromatographic separation was performed using UFLC-DAD connected with Ascentis Express Biphenyl column (system I) and Ascentis Express F5 pentafluorophenylpropyl (PFP) column (system II). Multistep gradient elution program was used in both analytical instruments to detect 34 compounds of different classes (phenolic acids, flavonoids, isoflavones, flavanols, flavonols and flavanones). The detection wavelength, linear calibration range, theoretical plate, tailing factor, limits of detection and of quantification were determined. The results were validated and the optimized method was proven to be precise, revealing good separation in the UFLC system II using Ascentis Express F5 PFP column. In conclusion, this study shows analytical preciseness and is useful in determining polyphenols in natural products. [GRAPHICS] .
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