期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 198, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2019.110714
关键词
Cytotoxic gold complexes; Metallodrug; Prodrug; Electrochemical cell; Voltammetry; Electrospray MS
资金
- Warsaw University of Technology
- Polish National Center of Science (NCN) [DEC-2013/09/B/ST4/00961]
A micro-electrochemical reaction cell was coupled to an electrospray mass spectrometer in order to track redox transformations for two representative medicinal gold compounds-Le. {(2,3,4,6-tetra-O-acetyl-1-thio-beta-D-glucopyranosato-S)(triethylphosphine)gold(D] and [Au(bipy(dmb)-H)(OH)][PF6] (where bipy(dmb)-H is deprotonated 6-(1,1-dimethylbenzy1)-2,2'-bipyridine), known as Auranofin and Aubipy(c) respectively-in parallel to square wave voltammetry (SWV) measurements. Irreversible oxidation of thio-glucose tetraacetate was the dominant reaction for the gold(I) compound Auranofin; oxidation was accompanied by hydrolysis leading to progressive deacetylation. Two main active forms were identified for this prodrug: the triethylphosphinegold(I) cation and a gold(I) thioglucose species, with a variable number of acetyl groups. For the gold(III) complex Aubipy(c) irreversible reduction of the gold(III) center was highlighted, accompanied by a ligand exchange process. The free gold(I) ion is proposed to be the final species that subsequently binds transport proteins in the bloodstream. Molecule specific mass spectrometry determinations provide complementary data to square wave voltammetry helping to understand the nature of the electrochemical conversions of complex or unstable compounds. Finally, it was possible to establish that oxidizing conditions during drug preparation and administration should be avoided in the case of Auranofin; conversely, reduction conditions typical for the blood or the cytosol environment are suitable to obtain the active gold(I) species from the gold(III) complex Aubipy(c).
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