期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 194, 期 -, 页码 223-232出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2019.01.016
关键词
Cu(II) complexes; Metallonucleases; Bis(2-benzimidazolyl) ligands; DNA cleavage; Reactive oxygen species; DNA binding
Cu(II) complexes of bis(2-benzimidazolyl) ligands connected by different linker moieties (disulfide, ethylene, ortho-phenylene) were applied in DNA cleavage reactions. Hydroxyl radicals and hydrogen peroxide were proven as reactive oxygen species (ROS) in a DNA quenching experiment. Thus, an oxidative DNA cleavage mechanism is suggested. The binding affinity of the Cu(II) complexes to DNA was studied by UV-VIS (DNA melting), fluorescence (ethidium bromide displacement assay) and circular dichroism (CD) spectroscopy indicating a correlation between DNA binding and DNA cleavage efficiency. The most important finding was that oxidative nuclease activity correlated with flexibility of the linker between the benzimidazole moieties. A more flexible linker allowed for an easier switch between square planar (Cu(II)) and tetrahedral geometry (Cu(I)) for the complex, and thus resulted in an enhanced ROS generation. EPR spectroscopy and cyclic voltammetry were applied to investigate such changes in geometry and redox state.
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