期刊
JOURNAL OF IMMUNOLOGY
卷 203, 期 1, 页码 76-83出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1801625
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- Italian Ministry of Health
The immune regulatory mechanisms that modulate Th1 and Th17 immune responses are altered in multiple sclerosis (MS). The inhibitory TIM-3/Gal-9 pathway, in particular, is impaired in primary progressive MS (PPMS). Recent results showed that carcinoembryonic Ag-related cell adhesion molecule 1 (CEACAM-1), a molecule expressed on activated T lymphocytes, endows TIM-3 with inhibitory function and facilitates the maturation and cell surface expression of TIM-3. We analyzed by flow cytometry CEACAM-1 expression on myelin basic protein (MBP)-stimulated CD4(+) and CD8(+) T lymphocytes of 56 MS patients with a diagnosis of either PPMS (n = 16), relapsing-remitting MS (n = 20), or benign MS (n = 20) and 40 age- and sex-matched healthy controls. The expression of TIM-3 and annexin V (AV) as well as the production of IFN-gamma and the intracellular concentration of HLA-B-associated transcript 3 (Bat3), a molecular adaptor that binds the intracellular tail of TIM-3 promoting both proliferation and proinflammatory cytokine production, were analyzed as well in the same cells. Results showed the following in PPMS: 1) CD4(+)/CEACAM-1(+), CD4(+)/TIM-3(+), CD8(+)/TIM-3(+), CD4(+)/CEACAM-1(+)/TIM-3(+), and CD8(+)/CEACAM-1(+)/TIM-3(+) T lymphocytes as well as CEACAM-1 mean fluorescence intensity on CD4(+) T lymphocytes were significantly reduced; 2) apoptotic CD4(+)/AV(+)/CEACAM-1(+) and CD8(+) /AV(+)/CEACAM-1(+) T lymphocytes were significantly reduced; and 3) Bat3-expressing CD4(+) and CD8(+) T cells were significantly increased. Notably, a specular immunologic scenario was seen in benign MS. CEACAM-1 expression is reduced in PPMS; this exacerbates MBP-specific inflammatory T cell response and reduces the apoptosis of MBP-specific T lymphocytes, possibly as a consequence of the upregulation of Bat3 seen in these patients.
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