期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 368, 期 -, 页码 514-522出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2019.01.079
关键词
Polyhalogenated carbazoles; 2,7-Dibromocarbazole; Transgenic zebrafish; Transcriptomics; Cardiac teratogenic effect
资金
- National Nature Science Foundation of China [21677130, 2137005, 21707121]
Polyhalogenated carbazoles (PHCZs), which have the similar structure of dioxin, have been reported ubiquitous in the environments and drawn wide concerns. However, their potential ecological and health risks are still poorly understood. Here, wildtype zebrafish embryos were used to evaluate the environmental risks of 2,7-dibromocarbazole (2,7-DBCZ), 3,6-dibromocarbazole (3,6-DBCZ), and 3,6-dichlorocarbazole (3,6-DCCZ). 2,7-DBCZ was the most toxic compound with the 96-h LC50 value of 581.8 +/- 29.3 mu g.L-1 and the EC50 value of 201.5 +/- 6.5 mu g.L-1 for pericardial edema. The teratogenic effects of 2,7-DBCZ were tested using transgenic zebrafish larvae. The transcriptomic analysis revealed that 90 genes in zebrafish expressed differently after exposure to 2,7-DBCZ, and many pathways were related to aryl hydrocarbon receptor (AhR) activation. The qRT-PCR also showed that expression levels of AhR1 and CYP1 A in zebrafish were significantly up-regulated after exposure to 2,7-DBCZ. In conclusion, 2,7-DBCZ exhibited more potent toxicity and cardiac teratogenic effects, and presented developmental toxicity partially consistent with AhR activation. Our results will be of great help to the risk assessment and regulation-making of PHCZ5. Meanwhile, further studies should be promoted to illustrate the potential mechanism between PHCZ5 and AhR in the near future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据