期刊
JOURNAL OF FOOD SCIENCE
卷 84, 期 5, 页码 1201-1207出版社
WILEY
DOI: 10.1111/1750-3841.14501
关键词
apigenin; bioactive compounds; biological activities; kaempferol; subcritical water extraction
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2016R1D1A1B03936128]
- National Research Foundation of Korea [2016R1D1A1B03936128] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The present study investigated the recovery of antioxidative compounds obtained from Pseuderanthemum palatiferum (Nees) Radlk. and their biological activities using subcritical water in comparison with hot water, Soxhlet, and methanol extraction. In this study, high-performance liquid chromatography (HPLC) and three commonly used assays, that is, 2,2-diphenyl-1-picrylhydrazyl assays, 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power, were employed to measure the number of valuable compounds and their antioxidant capacities, respectively. Additionally, the antimicrobial ability of the various extracts against seven pathogenic bacteria strains was assessed. The results demonstrated that the extracts obtained from subcritical water extraction (SWE) contain the highest amount of saponin (33.82 +/- 2.08 mg diosgenin/g), phenolic (34.87 +/- 0.29 mg CE/g), and protein (104.66 +/- 7.63 mg BSA/g), which are obtained at 170 degrees C, 190 degrees C, and 230 degrees C, respectively. The highest amounts of total sugar and flavonoids (211.73 +/- 1.58 mg glucose/g and 20.71 +/- 0.42 mg RE/g, respectively) were obtained at lower temperature (130 degrees C and 130 degrees C, respectively), and these values were higher than those obtained from hot water, Soxhlet, and methanol extraction. HPLC analysis indicated that the highest amount of apigenin (3.46 +/- 0.03 mg/g) and kaempferol (2.43 +/- 0.03 mg/g) were obtained by SWE at 170 degrees C and 190 degrees C, respectively. Furthermore, the extracts from SWE exhibited higher antimicrobial activity against five of the seven pathogenic bacterial strains tested compared with those obtained from conventional extraction methods. Therefore, subcritical water could be utilized as a cost-effective and green solvent to extract valuable compounds from P. palatiferum (Nees) Radlk. leaf. Practical Application Subcritical water was able to recover more bioactive compounds from the sample, such as phenolics, flavonoids, saponin, protein, and polysaccharides, than conventional solvents. The mixture of polyphenolic-polysaccharide-protein conjugates could be used in further steps, isolation, and purification, and applied to functional food.
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