期刊
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
卷 81, 期 -, 页码 46-54出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2019.05.007
关键词
Tea; Free amino acids; AQC derivatives; UHPLC-MS/MS; Principal component analysis
资金
- Postdoctoral Science Foundation of China [2018M63075]
- Science and Technology Planning Project of Fujian Province [2018Y0018]
- Open Science Project for Key Laboratory of Tea Science in Fujian Provincial University [KLTS 2018001]
- Fujian Provincial Special Project of Chinese Oolong Tea Industry Innovation Center [J2015-75]
6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) pre-column derivatization coupled with reversed-phase liquid chromatography and diode array detection (RPLC-DAD) is one of the conventional methods for analysis of free amino acids in tea. In this article, we improved method detection sensitivity and resolution by replacing the optical detector with a mass spectrometer (MS). We also optimized the extraction process and mobile phases. Application of mass spectrometry makes co-eluting compounds easier to separate. The improved method was then successfully applied in the analysis of twenty-eight commercial tea samples with various degrees of fermentation. Umami-tasting AAs were higher in green tea than in white tea, while sweet-tasting AAs were more elevated in white tea. Principal component analysis indicated that theanine, arginine, aspartic acid and glutamic acid could be potent discriminators for tea fermentation degree.
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