期刊
JOURNAL OF FOOD BIOCHEMISTRY
卷 43, 期 7, 页码 -出版社
WILEY
DOI: 10.1111/jfbc.12863
关键词
amino acid metabolism; proteome; storage; tartary buckwheat; two-dimensional liquid chromatography
资金
- National Natural Science Foundation of China [31701358, 31771716]
- Earmarked Fund for Modern Agro-industry Technology Research System [CARS-08-02A]
- Research Program of Sichuan Science and Technology Department [2018CC0021]
The whole seed of tartary buckwheat (Fagopyrum tataricum) is considered as a healthy and functional food, which is rich in kinds of flavonoids and with potential antioxidant effect. An in-depth analysis of tartary buckwheat seed (TBS) proteome was performed using a shotgun proteomics strategy. Total protein of TBS was extracted and digested, then the peptides were separated by offline two-dimensional liquid chromatography and identified by tandem mass spectrometry. Total of 3,363 high-confidence proteins were identified from 13,730 matched peptides, in which, 2,499 proteins were annotated by the Gene Ontology (GO) analysis with 1,720 involved in biological process, 2,241 in molecular function, and 693 in cellular components. Based on the GO functional enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment results, buckwheat seed proteins were mostly enriched in metabolism of nucleic acid, respiration and energy metabolism, as well as synthesis and metabolism of protein. Practical applications This study characterized the tartary buckwheat seed proteome on a scale of 3,000+ proteins and provide important information and clues for future research, especially in the mechanism of seed germination, nutrient composition changes, and metabolite production seed germination and material metabolism.
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