期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1599, 期 -, 页码 9-16出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2019.04.005
关键词
Phytoestrogen; Molecularly imprinted polymer; Supported liquid membrane; Solid-Phase extraction; Urine
资金
- Spanish Ministry of Economy and Competitiveness [CTQ2011-24807]
An efficient sample clean-up and preconcentration procedure for phytoestrogens analysis in urine has been developed. It was based on a combination of solid phase extraction with hollow-fiber supported liquid membrane and molecularly imprinted beads (MIPs-HF-SLM-SPE). The molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization technique with biochanin A (BCA) as a template, giving narrowly dispersed microspheres with a regular shape. As the functional monomer, (dimethylamino)ethyl methacrylate (-DEM) turned out to be better than methacrylic acid (MAA) to get the best-imprinted effects. The MIPs used as sorbents in the MIPs-HF-SLM-SPE extraction process exhibited excellent binding selectivity for BCA, in comparison to non-imprinted polymers as well as its structural analogs (genistein and daidzein). Finally, the developed method was used to detect BCA in urine. Under optimal extraction conditions, the recovery of BCA in urine samples (using 4.5 mL sample spiked with 10 mu g L-1) was over 41%, with a coefficient of variation (CV) < 6.6% (n = 5). The detection limit (LOD) and quantification limit (LOQ) for BCA analysis in urine were 0.41 and 1.36 mu gL(-1), respectively. (C) 2019 Elsevier B.V. All rights reserved.
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