期刊
JOURNAL OF CELL SCIENCE
卷 132, 期 10, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.226019
关键词
Tyrosine phosphorylation; STIM1; Orai1; Y-316; I-CRAC; SARAF
类别
资金
- Ministerio de Economia y Competitividad (MINECO) [BFU2013-45564C2-1-P, BFU2016-74932-C2]
- Consejeria de Educacion y Empleo, Junta de Extremadura - FEDER [GR18061, IB16046]
- Instituto de Salud Carlos III (ISCIII) [FI10/00573]
- Junta de Extremadura
- Austrian Science Fund (FWF) [P28872]
- Austrian Science Fund (FWF) [P28872] Funding Source: Austrian Science Fund (FWF)
Stromal interaction molecule 1 (STIM1) is one of the key elements for the activation of store-operated Ca2+ entry (SOCE). Hence, identification of the relevant phosphorylatable STIM1 residues with a possible role in the regulation of STIM1 function and SOCE is of interest. By performing a computational analysis, we identified that the Y-316 residue is susceptible to phosphorylation. Expression of the STIM1-Y316F mutant in HEK293, NG115-401L and MEG-01 cells resulted in a reduction in STIM1 tyrosine phosphorylation, SOCE and the Ca2+ release-activated Ca2+ current (I-CRAC). STIM1-Orai1 colocalization was reduced in HEK293 cells transfected with YFP- STIM1-Y316F compared to in cells with wild-type (WT) YFP-tagged STIM1. Additionally, the Y316F mutation altered the pattern of interaction between STIM1 and SARAF under resting conditions and upon Ca2+ store depletion. Expression of the STIM1 Y316F mutant enhanced slow Ca2+-dependent inactivation (SCDI) as compared to STIM1 WT, an effect that was abolished by SARAF knockdown. Finally, in NG115-401L cells transfected with shRNA targeting SARAF, expression of STIM1 Y316F induced greater SOCE than STIM1 WT. Taken together, our results provide evidence supporting the idea that phosphorylation of STIM1 at Y-316 plays a relevant functional role in the activation and modulation of SOCE.
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