期刊
JOURNAL OF CELL SCIENCE
卷 132, 期 11, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.227033
关键词
DnaJB6; RanGTP; Spindle organization; Dynein; Microtubules
类别
资金
- Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa'
- CERCA Programme/Generalitat de Catalunya
- FPI fellowship from the Spanish Ministry of Economy [BES-2013-064601]
- Spanish Ministry of Economy and Competitiveness I+D [BFU2012-37163, BFU2015-68726-P]
Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.
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