期刊
JOURNAL OF CELL BIOLOGY
卷 218, 期 7, 页码 2215-2231出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201805002
关键词
-
类别
资金
- Centre National de la Recherche Scientifique
- Fondation pour la Recherche Medicale [FRM DEQ20120323723, FRM FDT20150532154]
- Labex CellTisPhyBio
- Agence Nationale de la Recherche [ANR-12-BSV2-0003-01]
- European Research Council [339847 MYODYN]
- ARC
- Institut Curie
- Agence Nationale de la Recherche
To ensure their homeostasis and sustain differentiated functions, cells continuously transport diverse cargos to various cell compartments and in particular to the cell surface. Secreted proteins are transported along intracellular routes from the endoplasmic reticulum through the Golgi complex before reaching the plasma membrane along microtubule tracks. Using a synchronized secretion assay, we report here that exocytosis does not occur randomly at the cell surface but on localized hotspots juxtaposed to focal adhesions. Although microtubules are involved, the RAB6-dependent machinery plays an essential role. We observed that, irrespective of the transported cargos, most post-Golgi carriers are positive for RAB6 and that its inactivation leads to a broad reduction of protein secretion. RAB6 may thus be a general regulator of post-Golgi secretion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据