期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 31, 期 5, 页码 3131-3141出版社
SPRINGER
DOI: 10.1007/s10811-019-01792-y
关键词
Padina tetrastromatica; Phaeophyceae; Phlorotannin; Mass spectrometry; DPPH assay; Anti-inflammatory
资金
- Amrita Vishwa Vidyapeetham
In this study, LC-MS/MS-based structural characterisation of phlorotannins from Padina tetrastromatica, a marine brown macroalga collected from South-West coastal region of Kerala, and its bioactivities are presented. The tandem mass spectrometric data revealed a series of phlorotannins with degree of polymerisation ranging from 2 to 18. The characteristic neutral loss of tandem mass spectra further confirmed that these molecules belong to fucophlorethol class of phlorotannins. DPPH assay of the HPLC-purified, phlorotannin-enriched fraction possesses significant free radical-scavenging activity. Cell viability assay indicated that phlorotannin concentration ranging from 1.5 to 50.0 mu g mL(-1) is non-toxic to THP-1 cell lines. Anti-inflammatory assay performed through gelatin zymography confirmed that phlorotannins ameliorated high-glucose-induced pro-MMP-9 expression in a dose-dependent manner whereas the level of pro-MMP-2 remains unaltered. The antimicrobial assays carried out using both the crude and HPLC-purified phlorotannin fraction showed its anti-MRSA potential.
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