期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 22, 页码 6313-6323出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b00569
关键词
gliadins; allergenicity; protein structure
资金
- Fundamental Research Funds for the Central Universities [JUSR-P51708A, JUSRP11842]
- National Natural Science Foundation of China [31671890]
- Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment Technology [FMZ201807]
- National first-class discipline program of Food Science and Technology [JUFSTR20180103]
- Ministry of Science and Technology of China [2016YFA0500102, 2016YFC1304905]
- Major Science and Technology Projects in Anhui Province [18030701176]
Gliadins are major allergens responsible for wheat allergies. Food processing is an effective strategy to reduce the allergenicity of gluten. In the present study, we determined the secondary and tertiary structures of gluten and gliadins treated by chemical, physical, and enzymatic means through FTIR, surface hydrophobicity, intrinsic fluorescence spectra, and UV absorption spectra. The results showed that the three treatments of phosphorylation and alcalase and papain hydrolyses significantly changed the conformational structures of gliadins, especially the secondary structure. Then, the potential allergenicity of the phosphorylated and alcalase and papain hydrolyzed gliadins were further characterized, and we observed a significant decrease in the allergenicity through the results of the index of spleen, serum total IgE, gliadin-specific IgE, histamine, and serum cytokine concentrations. An elevation of Th17 cells, the absence of Treg cells, and an imbalance in Treg/Th17 are associated with allergy. On the basis of the expression levels of related cytokines and key transcription factors, we also confirmed that phosphorylation and alcalase and papain hydrolysis could effectively reduce the allergenicity of gliadins by improving the imbalance of both Th1/Th2 and Treg/Th17 in the spleens of sensitized mice. This study suggested that the changes in conformational structure contribute to gliadin hyposensitization and that phosphorylation and alcalase and papain hydrolysis may be promising strategies for the production of wheat products with low allergenicity.
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