Susceptibility profile of Candida rugosa (Diutina rugosa) against antifungals and compounds of essential oils

Candida rugosa (recently reclassified Diutina rugosa) is an emerging pathogen affecting humans and animals. Candida resistance to existing drugs is an important factor to be monitored, as well as the need of researching alternatives to conventional antifungals. Here, we evaluated the in vitro effects of some antifungals and major components of essential oils by the broth microdilution method (CLSI M27-A3) against fifteen C. rugosa strains from animals isolated and molecular identificated. The results showed MIC90 of: 0.125 mu g/mL to ketoconazole and voriconazole, 0.25 mu g/mL to micafungin, 0.5 mu g/mL to anidulafungin, 1 mu g/mL to caspofungin, 2 mu g/mL to amphotericin B, itraconazole and flucytosin, 8 mu g/mL to fluconazole, 16 mu g/mL to nystatin and >128 mu g/mL to terbinafine. The compounds carvacrol (MIC90 320 mu g/mL), thimol (MIC90 320 mu g/mL) and cinnamaldehyde (MIC90 160 mu g/mL) demonstrated antifungal activity against the samples tested. (C) 2019 Elsevier Masson SAS. All rights reserved.