期刊
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
卷 69, 期 7, 页码 1986-1992出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.003412
关键词
Azotobacter bryophylli sp. nov.; Bryophyllum pinnatum; diazotroph; novel species; nitrogen-fixation
类别
资金
- National Natural Science Foundation of China [31370052]
- Science and Technology Program of Guangdong Province [2014A050503058, 2014A030313459, 2016B020242005, 2018A050506075]
- Guangdong Province Key Laboratory of Plant Molecular Breeding
- Guangdong Innovative Research Team Program [2013S033]
A Gram-stain-negative, aerobic, nitrogen-fixing bacterium, designated strain L461(T), was isolated from leaves of Bryophyllum pinnatum growing at the South China Agricultural University. Phylogenetic analysis of the 16S rRNA gene sequence indicated it as a member of the genus Azotobacter closely related to Azotobacter beijerinckii JCM 20725(T) (97.82 % similarity) and Azotobacter chroococcum ATCC 9043(T) (97.34 %). Its major fatty acid components were C-16:1 omega 9c and C-16:0. Its predominant isoprenoid quinone was Q-9. Its major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid, phospholipid and one unknown lipid. Its DNA G+C content was 64.9 mol% (Tm). DNA-DNA relatedness values between strain L461(T) and the reference strains of A. beijerinckii and A. chroococcum were 46.43 and 28.23%, respectively. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting, and comparison of cellular fatty acids distinguished strain L461(T) from the closely related Azotobacter species. Based on these data, the novel species Azotobacter bryophylli sp. nov. is proposed, with the type strain L461(T) (=KCTC 62195(T)=GDMCC 1.1250(T)).
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