期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 20, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/ijms20102602
关键词
polyphenol oxidase; MnMYB3R; yeast one-hybrid
资金
- National Key Research and Development Program of China [2018YFD1000602]
- National Natural Science Foundation of China [31572323]
- Project of extraction, detection and variation of alkaloids and polyphenols in Mulberry [cstc2016jcyjys0002]
The aim of this study was to determine how the mulberry (Morus notabilis) polyphenol oxidase 1 gene (MnPPO1) is regulated during plant stress responses by exploring the interaction between its promoter region and regulatory transcription factors. First, we analyzed the cis-acting elements in the MnPPO1 promoter. Then, we used the MnPPO1 promoter region [(1268 bp, including an MYB3R-binding cis-element (MSA)] as a probe to capture proteins in DNA pull-down assays. These analyses revealed that the MYB3R1 transcription factor in M. notabilis (encoded by MnMYB3R1) binds to the MnPPO1 promoter region. We further explored the interaction between the MnPPO1 promoter and MYB3R1 with the dual luciferase reporter, yeast one-hybrid, and chromatin immunoprecipitation assays. These analyses verified that MnMYB3R1 binds to the MSA in the MnPPO1 promoter region. The overexpression of MnMYB3R1 in tobacco upregulated the expression of the tobacco PPO gene. This observation as well as the quantitative real-time PCR results implied that MnMYB3R1 and PPO are involved in the abscisic acid-responsive stress response pathway.
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