期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 129, 期 -, 页码 246-253出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2019.01.163
关键词
Juniperus pingii var. wilsonii; Polysaccharides; Acute lung injury; Complement inhibition; Structure characterization
资金
- National Natural Science Foundation of China [81673690, 81330089, 81872977]
- Ministry of Science and Technology of the People's Republic of China [2018ZX09735003-002]
- major project of Science and Technology of Tibet Autonomous Region, China [XZ201801-GH-13]
Juniperus pingii var. wilsonii has been traditionally used in Tibetan medicine for the treatment of inflammatory diseases. In the present study, J. pingii var. wilsonii polysaccharides (JPWP), with high content of D-galacturonic acid, showed potent anti-complementary activity in vitro and significantly attenuated acute lung injury (ALI) induced by H1N1 influenza virus in vivo through reducing the inflammatory responses, alleviating oxidative stress and inhibiting the activation of complement. Thus, anti-complementary activity-guided fractionation of JPWP led to the isolation of an acidic homogeneous polysaccharide, JPWP-PS, whose structure was further elucidated by acid hydrolysis, PMP derivation, methylation and NMR analysis. JPWP-PS had potent anti-complementary activity with the CH50 value of 0.073 +/- 0.009 mg/mL, and was characterized by the residues of T-Araf-(1 ->, -> 3)-Araf-(1 ->, -> 3,5)-Araf-(1 ->, -> 3)-Galp-(1 -> and -> 4)-GalpA-(1 ->. (C) 2019 Elsevier B.V. All rights reserved.
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