期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 111, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2019.05.006
关键词
Bumblebee; Neonicotinoids; Cytochrome P450; Metabolism
资金
- Bayer AG
- European Research Council under the European Union's Horizon 2020 research and innovation programme [646625]
- Biotechnology and Biological Sciences Research Council (BBSRC) [15076182]
- Biotechnology and Biological Sciences Research Council's Industrial Strategy Challenge Fund [BBS/OS/CP/000001]
- European Research Council (ERC) [646625] Funding Source: European Research Council (ERC)
Recent work has shown that two bumblebee (Bombus terrestris) cytochrome P450s of the CYP9Q subfamily, CYP9Q4 and CYP9Q5, are important biochemical determinants of sensitivity to neonicotinoid insecticides. Here, we report the characterisation of a third P450 gene CYP9Q6, previously mis-annotated in the genome of B. terrestris, encoding an enzyme that metabolises the N-cyanoamidine neonicotinoids thiacloprid and acetamiprid with high efficiency. The genomic location and complete ORE of CYP9Q6 was corroborated by PCR and its metabolic activity characterised in vitro by expression in an insect cell line. CYP9Q6 metabolises both thiacloprid and acetamiprid more rapidly than the previously reported CYP9Q4 and CYP9Q5. We further demonstrate a direct, in vivo correlation between the expression of the CYP9Q6 enzyme in transgenic Drosophila melanogaster and an increased tolerance to thiacloprid and acetamiprid. We conclude that CYP9Q6 is an efficient metaboliser of N-cyanoamidine neonicotinoids and likely plays a key role in the high tolerance of B. terrestris to these insecticides.
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