期刊
IMMUNOLOGICAL INVESTIGATIONS
卷 48, 期 6, 页码 644-658出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/08820139.2019.1610888
关键词
Kidney transplantation; soluble HLA-G; 3 ' UTR region; gene polymorphism; rejection
类别
资金
- VEGA from the Scientific Grant Agency of Ministry of Education of Slovak Republic
- Slovak Academy of Sciences [2/0080/15]
Background: Human leukocyte antigen G (HLA-G) belongs to nonclassical HLA I molecule involving in the suppression of immune response. Besides its profound effect to induce fetal tolerance, HLA-G expression has been associated with allograft acceptance. For the regulation of HLA-G levels, polymorphic sites within the 3 ' untranslated region (3 ' UTR) are of crucial importance. The aim of the study was to analyze the association between several HLA-G 3 ' UTR variants (+3003T/C, +3010C/G, +3027C/A, +3035C/T, +3142G/C, +3187A/G, and +3196C/G), soluble HLA-G (sHLA-G) level, and kidney graft outcome in the Slovak Caucasian population. Methods: We investigated 69 kidney transplant recipients (45 males, 24 females) of age 27-65 years. Out of this group, 37 recipients developed acute rejection that was biopsy proven. Recipient's plasma was obtained at 1 day before transplantation and analyzed by ELISA. The HLA-G 3 ' UTR polymorphisms were typed by direct sequencing. Results: In the recipients with stable allograft function, significantly higher values of sHLA-G were found in the homozygous +3010GG, +3142CC, +3187GG, and +3196CC carriers in comparison to the acute rejection recipients (P = 0.01-0.05). Conclusion: The study demonstrated genetic association between HLA-G 3 ' UTR variants and sHLA-G level in kidney recipients leading to graft acceptance. We suggest to monitor the pretransplantation sHLA-G level as additional marker to predict kidney graft outcome.
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