期刊
IET NANOBIOTECHNOLOGY
卷 13, 期 6, 页码 602-608出版社
WILEY
DOI: 10.1049/iet-nbt.2018.5407
关键词
nanofabrication; ultraviolet spectra; catalysis; molecular biophysics; enzymes; dyes; platinum; electrokinetic effects; transmission electron microscopy; particle size; X-ray diffraction; visible spectra; pH; nanomedicine; nanoparticles; biochemistry; light scattering; scanning electron microscopy; Fourier transform infrared spectra; reduction (chemical); oxidation; antioxidant activities; catalytic action; salt concentration; artificial peroxidase; two-fold enhancement; PCR yield; multifaceted activities; plant gum synthesised platinum nanoparticles; gum ghatti; anogeissus latifolia; analytical characterisation tools; dynamic light scattering; zeta potential; X-ray diffraction; XRD; black solution colouration; Pt NP formation; face-centred cubic crystalline structure; peroxidase activity; ultraviolet-visible spectroscopy; transmission electron microscopy; Fourier transform infrared spectroscopy; particle size; catalytic activity; PCR enhancing activity; single pot green method; wide continuous UV-visible absorption; polymerase chain reaction enhancing activity; arsenazo-III; azo dye decolourisation; 3; 3 '; 5; 5 '-tetramethylbenzidine oxidation; pH; environmental conditions; 1 '; 1 ' diphenyl picryl-hydrazyle scavenging; time 0; 0 month to 12; 0 month; temperature 25; 0 degC to 100; 0 degC; Pt
A single pot, green method for platinum nanoparticles (Pt NP) production was devised with gum ghatti (Anogeissus latifolia). Analytical tools: ultraviolet-visible (UV-vis), dynamic light scattering, zeta potential, transmission electron microscope, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy were employed. Wide continuous UV-vis absorption and black solution colouration proved Pt NP formation. Face-centred cubic crystalline structure of NP was evidenced from XRD. NPs formed were nearly spherical with a mean particle size of 3 nm. NP demonstrated a myriad of properties including catalytic, peroxidase, polymerase chain reaction (PCR) enhancing and antioxidant activities. Catalytic action of NP was probed via NaBH4 reduction of arsenazo-III dye. NP displayed considerable peroxidase activity via catalysis of 3, 3 ', 5, 5 '-tetramethylbenzidine oxidation by H2O2. NP showed exceptional stability towards varying pH (3-11), temperature (25-100 degrees C), salt concentration (0-100 mM) and storage time duration (0-12 months). In comparison with horse radish peroxidase, its applicability as an artificial peroxidase is advantageous. NP caused a two-fold enhancement in PCR yield at 0.4 nM. Also showed significant 1 ', 1 ' diphenyl picryl-hydrazyle scavenging (80.1%) at 15 mu g/mL. Author envisages that the biogenic Pt NP can be used in a range of biological and environmental applications.
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