期刊
IEEE TRANSACTIONS ON NANOBIOSCIENCE
卷 18, 期 2, 页码 265-268出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2019.2905517
关键词
Biological cells; composite materials; stereolithography; three-dimensional printing
资金
- NSF Faculty Early Career Development (CAREER) Program [BBBE 1254608]
- NIH [P20GM103432]
- University of Wyoming School of Energy Resources Graduate Assistantship
Advancements in the microfabrication of soft materials have enabled the creation of increasingly sophisticated functional synthetic tissue structures for a myriad of tissue engineering applications. A challenge facing the field is mimicking the complex microarchitecture necessary to recapitulate proper morphology and function of many endogenous tissue constructs. This paper describes the creation of PEGDA hydrogel microenvironments (microgels) that maintain a high level of viability at single cell patterning scales and can be integrated into composite scaffolds with tunable modulus. PEGDA was stereolithographically patterned using a digital micromirror device to print single cell microgels at progressively decreasing length scales. The effect of feature size on cell viability was assessed and inert gas purging was introduced to preserve viability. A composite PEGDA scaffold created by this technique was mechanically tested and found to enable dynamic adjustability of the modulus. Together this approach advances the ability to microfabricate tissues that better mimic native constructs on cellular and subcellular length scales.
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