4.7 Article

Structure-Based Intensity Propagation for 3-D Brain Reconstruction With Multilayer Section Microscopy

期刊

IEEE TRANSACTIONS ON MEDICAL IMAGING
卷 38, 期 5, 页码 1106-1115

出版社

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TMI.2018.2878488

关键词

Brain reconstruction; confocal microscopy; registration

资金

  1. Presidential Fellowship for Collaborative Neuroscience, University of Virginia
  2. NIH [R01 NS040337-13, R01 NS044370-12, R21 EB022309]
  3. Thomas F. and Kate Miller Jeffress Memorial Trust, Bank of America, Trustee

向作者/读者索取更多资源

Microscopy is widely used for brain research because of its high resolution and ability to stain for many different biomarkers. Since whole brains are usually sectioned for tissue staining and imaging, reconstruction of 3D brain volumes from these sections is important for visualization and analysis. Recently developed tissue clearing techniques and advanced confocal microscopy enable multilayer sections to be imaged without compromising the resolution. However, noticeable structure inconsistence occurs if surface layers are used to align these sections. In this paper, a structure-based intensity propagation method is designed for the robust representation of multilayer sections. The 3D structures in reconstructed brains are more consistent using the proposed methods. Experiments are conducted on 367 multilayer sections from 20 mouse brains. The average reconstruction quality measured by the structure consistence index increases by 45% with the tissue flattening method and 29% further with the structure-based intensity propagation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据