期刊
HUMAN PATHOLOGY
卷 87, 期 -, 页码 18-27出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.humpath.2019.02.002
关键词
Colorectal cancer; Tumor budding; Cancer-associated stroma; Epithelial-mesenchymal transition; Mesothelial-mesenchymal transition
类别
资金
- Werner and Hedy Berger Janser Stiftung
- Mach-Gaensslen Stiftung
- Johanna Durmuller-Bol foundation
Tumor buds in colorectal cancer are hypothesized to undergo a (partial) epithelial-mesenchymal transition (EMT). If so, cytokeratin (CK) and vimentin (VIM) co-expression is expected. CK+/VIM+ can also be found in some stromal cells; however, their origin remains unclear. Here, we determine the frequency of CK+/VIM+ tumor cells and characterize the CK+/VIM+ stoma in colorectal cancer. Three cell populations (CK+, VIM+, CK+/VIM+) were sorted using DepArray and fluorescence-activated cell sorting (FACS). Tumor areas were selected to include tumor center, stroma and tumor budding. Fluorescence microscopy was used to visualize co-expressing cells on whole slides. A next-generation tissue microarray (ngTMA) of matched Pan-CK positive and-negative stroma was constructed and stained for E-cadherin, VIM, Snail 1, Twist1, Zeb1 and Zeb2, COL11A1, SPARC, CD90, alpha-SMA, FAP and WT1. CK+/VIM+ co-expressing tumor cells were detected using all three methods. With DepArray, only tumor budding areas contained CK+/VIM+ cells. The proportion of CK+/VIM+ tumor cells was low (1.5%-22%). CK+ stroma was associated with aggressive tumor features like distant metastasis (P =.0003), lymphatic invasion (P =.0009) and tumor budding (P =.0084). CK+/VIM+ stroma was characterized by positive WTI (P <.001), ZEB2 (P <.001), TWIST1 (P =.009), and FAP (P =.003). Our data suggest that CK+/VIM+ tumor cells exist, albeit in low numbers and could represent a subgroup of tumor buds in partial EMT. CK+/VIM+ stroma may be of mesothelial origin and shows features of mesenchymal cells and cancer-associated fibroblasts. These results, together with the association with metastasis point to cells in mesothelial-mesenchymal transition (MMT). This atypical stroma may be a potential target for therapy. (C) 2019 Elsevier Inc. All rights reserved.
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