期刊
GLIA
卷 67, 期 8, 页码 1558-1570出版社
WILEY
DOI: 10.1002/glia.23628
关键词
cell proliferation; G protein-coupled receptors and microtubules; glioblastomas
资金
- National Institute of General Medical Sciences [GM069429]
- National Institute of Neurological Disorders and Stroke [NS106924]
- National Institute on Drug Abuse [DA0144861]
- National Institutes of Health [GM069429, NS106924, DA0144861, NS099929, NS084217, AG051807, AG000538, AR065459]
GPR124 is involved in embryonic development and remains expressed by select organs. The importance of GPR124 during development suggests that its aberrant expression might participate in tumor growth. Here we show that both increases and decreases in GPR124 expression in glioblastoma cells reduce cell proliferation by differentially altering the duration mitotic progression. Using mass spectrometry-based proteomics, we discovered that GPR124 interacts with ch-TOG, a known regulator of both microtubule (MT)-plus-end assembly and mitotic progression. Accordingly, changes in GPR124 expression and ch-TOG similarly affect MT assembly measured by real-time microscopy in cells. Our study describes a novel molecular interaction involving GPR124 and ch-TOG at the plasma membrane that controls glioblastoma cell proliferation by modifying MT assembly rates and controlling the progression of distinct phases of mitosis.
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