期刊
FUTURE MICROBIOLOGY
卷 14, 期 6, 页码 509-518出版社
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2018-0297
关键词
biofilm; Candida parapsilosis; Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis
类别
资金
- Ministry of Health of the Czech Republic [16-29916 A, 16-31593 A]
- Czech Science Foundation [GA15-20645S]
- Ministry of Education, Youth and Sports of the Czech Republic [LO1212, MUNI/A/0955/2016]
- European Commission [CZ.1.05/2.1.00/01.0017]
- European Regional Development Fund [CZ.02.1.01/0.0/0.0/15003/0000476]
Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.
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