期刊
FOOD CHEMISTRY
卷 278, 期 -, 页码 197-202出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.11.071
关键词
Microcystin-LR; Surface enhanced Raman spectroscopy; Rapid detection; Food analysis; Magnetic nanoparticles
资金
- Nature Science Foundation of Jiangsu Province [BK20160168]
- Nature Science Foundation of China [31601413, 31501418]
- Special Funds for Taishan Scholars Project
We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200 ng/mL with the limit of detection (LOD) of 0.002 ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.
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