Synergistic effect of a combined live Vibrio anguillarum and Edwardsiella piscicida vaccine in turbot

In aquaculture, more than one pathogen usually be isolated from the sick fish, creating an urgent need for developing combined vaccines to control fish disease caused by multiple pathogens simultaneously. In our previous work, two live attenuated vaccines against Vibrio anguillarum and Edwardsienn piscicida were vaccinated in turbot, exhibiting an efficient protection. However, some immunological processes such as antigenic competition, antigenic cross-reaction and antigen induced suppression during combined vaccination are unknown. In this study, we evaluated the effectiveness of the combined live vaccines and explored the immunological processes after vaccination. We found that the combined two live attenuated vaccines for V. anguillarum and E. piscicida induced a stronger immune response without existing antigen competition. Instead, a synergistic effect was observed not only for triggering innate immune response but for stimulation of adaptive immunity. Our study suggested that the two combined live vaccines against V. anguillarum and E. piscicida could be used simultaneously in the future.