期刊
FISH & SHELLFISH IMMUNOLOGY
卷 88, 期 -, 页码 47-52出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.02.062
关键词
Litopenaeus vannamei; WSSV; Spirulina; Immunostimulation; Immune parameters; Gene expression; Intestinal immunity
资金
- Brazilian funding agency Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES (CIMAR) [1974/2014]
- Brazilian funding agency Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq (MEC/MCTI/CAPES/CNPq/FAPs PVE) [401191/2014-1]
- Brazilian funding agency Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq (MCTI/CNPq Universal) [406530/2016-5]
- CAPES
Herein, we evaluated the immunomodulatory and the antiviral protective properties of a cyanobacteria-enriched diet on the immune responses of the Pacific white shrimp Litopenaeus vannamei challenged with the White spot syndrome virus (WSSV). Shrimp were fed with an Arthrospira platensis supplemented feed during 20 days, and its effects were examined by evaluating well-known standardized shrimp immune parameters (total hemocyte counts, total protein concentration, phenoloxidase activity, and serum agglutination titer). Additionally, we assessed the expression of crucial genes involved in both hemolymph-and gut-based immunities related to the shrimp capacity to circumvent viral and microbial infections. Dietary supplementation improved shrimp survival rates after challenge with a median lethal dose of WSSV. From all immune parameters tested, only the serum agglutination titer was higher in treated animals. On the other hand, the expression of some representative marker genes from different immune response pathways was only modulated in the midgut and not in the circulating hemocytes, suggesting that this feed supplementation can be used as an attractive strategy to enhance immunity in shrimp gut. Altogether, our results evidence the immunomodulatory properties of A. platensis supplemented feed in shrimp humoral and intestinal defenses and highlight the potential use of cyanobacteriabased immunostimulants in shrimp farming for protection against infectious diseases.
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