期刊
FEBS LETTERS
卷 593, 期 9, 页码 942-951出版社
WILEY
DOI: 10.1002/1873-3468.13377
关键词
asparagine-linked glycans; extracellular vesicles; metastasis
资金
- SENSHIN Medical Research Foundation
- Kodama Memorial Fund for Medical Research
- MEXT/JSPS [JP17H06414, 17K07356]
- Grants-in-Aid for Scientific Research [17K07356] Funding Source: KAKEN
We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs). Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation. All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans. The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with beta 1,6-N-acetylglucosamine branches. A few N-glycans are extended by N-acetyllactosamine repeats. Sialylation of these N-glycans may generate cell-type-specific N-glycomes on EVs. Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据