期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 244, 期 10, 页码 824-833出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1535370219850084
关键词
Extracellular matrix; lipopolysaccharide; mesenchymal stem cell; vocal fold fibroblast; wound healing
资金
- Basic Science Research Program through the National Research Foundation - Ministry of Science, ICT, and Future Planning (MSIP), Republic of Korea [2017M3A9F7079339]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology, Republic of Korea [NRF-2015R1A1A1A05027697]
- National Research Foundation of Korea [2017M3A9F7079339] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Injection laryngoplasty is a widely used therapeutic option for drug delivery into vocal folds (VFs). Efficient injectable materials are urgently needed for treating intractable VF disease. Liquid-type non-thermal atmospheric plasma (LTP) has been found to be useful for various biological applications, including in regenerative medicine. We evaluated the effects of LTP on VF regeneration. Migration and matrix metalloproteinase-2 expression of lipopolysaccharide (LPS)-treated human vocal fold-derived mesenchymal stem cells (VF-MSCs) were enhanced by LTP treatment. LTP treatment not only ameliorated nuclear factor-kappa B and interleukin-6 activation, induced by LPS treatment, but also the increased manifestation of alpha-smooth muscle actin and fibronectin, induced by transforming growth factor-ss. In a rabbit VF scarring animal model, histological analyses showed increased hyaluronic acid deposition and decreased collagen accumulation after LTP injection. Videokymographic analysis showed more improved vibrations in LTP-treated VF mucosa compared to those in non-treated group. In conclusion, LTP treatment enhanced the recruitment and activation of VF-MSCs. Regulated extracellular matrix (ECM) synthesis and eventual functional improvement of scarred VFs were observed upon LTP treatment. The results of this study suggest that LTP injection can enhance wound healing and improve functional remodeling following VF injury.
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