期刊
ENVIRONMENTAL TOXICOLOGY
卷 34, 期 8, 页码 921-927出版社
WILEY
DOI: 10.1002/tox.22763
关键词
coronary artery disease; dilong; earthworm; H9c2 cells; KCI cardioplegia solution; lumbrokinase
资金
- Asia University, Taiwan [CMU103-ASIA-16]
- China Medical University, Taiwan
Hyperkalemia is often associated with cardiac dysfunction. In this study an earthworm extract (dilong) was prepared from dried Pheretima aspergillum powder and its effect against high-KCI challenge was determined in H9c2 cardiomyoblast cells. H9c2 cells pre-treated with dilong (31.25, 62.5, 125, and 250 mg/mL) for 24 hours, where challenged with different doses of KCI treatment for 3 hours to determine the protective mechanisms of dilong against cardiac fibrosis. High-KCI administration induced mitochondrial injury and elevated the levels of pro-apoptotic proteins. The mediators of fibrosis such as ERK, uPA, SP1, and CTGF were also found to be upregulated in high-KCI condition. However, dilong treatment enhanced IGF1R/PI3k/Akt activation which is associated with cell survival. In addition, dilong also reversed high-KCl induced cardiac fibrosis related events in H9c2 cells and displayed a strong cardio-protective effect. Therefore, dilong is a potential agent to overcome cardiac events associated with high-KCl toxicity.
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