Production of volatile fatty acids and H2 for different ratio of inoculum to kitchen waste

The aim of this study was to evaluate the effect of different inoculum ratio on the dark fermentation of kitchen waste in terms of volatile fatty acids (VFAs) and H-2 production. The experiments were performed in batch bioreactors of effective volume 1 L without pH regulation. The ratio between the DS and KW was being increased from 0.11 to 0.51 on a volatile solids (VS) basis, while the initial content of KW was equal to 34.1 g VS/L. Increase of the DS/KW ratio from 0.11 to 0.28 resulted in the rise of VFAs and H-2 production. Further increase in the amount of added DS did not cause a significant change in the production of VFAs and H-2. In the bioreactor with the DS/KW ratio of 0.28, the production of VFAs and H-2 was equal to 16.0 g/L and 68.1 mL/g VS, respectively. Acetic and butyric acids were produced in the largest amount and their content, for DS/KW ratio of 0.28, were equal 37% and 43%, respectively. At the ratio of DS/KW above 0.4, the caproic acid content attained the level of 25%. Based on the DS and KW microbiological analysis, it was observed that dominant bacteria were Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes and WWE1 at the phylum level.