期刊
ANNALS OF HEMATOLOGY
卷 95, 期 4, 页码 543-547出版社
SPRINGER
DOI: 10.1007/s00277-016-2608-z
关键词
Hematopoietic stem cell; Markers; Purification; Flow cytometry
类别
资金
- National Natural Science Foundation of China (NSFC) [31301161, 81270603, 81570201]
Lifelong production of blood cells is sustained by hematopoietic stem cells (HSC) which reside at a very low frequency within the bone marrow (BM). The use of flow cytometry has been critical in establishing methods to isolate and characterize HSCs and their progenitors. For more than 30 years, researchers have uncovered many novel markers that when used in combination significantly enhance the purification of HSCs from murine and human bone marrow. Here, we review the phenotypic markers and strategies used to purify HSCs.
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