期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 173, 期 -, 页码 339-346出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2019.01.116
关键词
Zebrafish; Marine sediments; cYP1A; AhR2 pathway; Bioassessment
资金
- National Natural Science Foundation of China [21267015, 21567019, 81360508, 41807352, 41877366, 641722110, 41571474]
- Inner Mongolia University for Nationalities, China [MDXK008]
- Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, China [MDK2017012, MDK2017014, MDK2018027]
- Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing, China [CSTCCXLJRC201714]
Bohai Bay, in the western region of northeastern China's Bohai Sea, receives water from large rivers containing various pollutants including dioxin-like compounds (DLCs). This study used the established zebrafish (Danio rerio) model, its known developmental toxicity endpoints and sensitive molecular analyses to evaluate sediments near and around an industrial effluent site in Bohai Bay. The primary objective was to assess the efficacy of rapid biological detection methods as an addition to chemical analyses. Embryos were exposed to various concentrations of sediment extracts as well as a 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) positive control. Exposure to sediment extract nearest the discharge site (P1) resulted in the most severe- and highest rates of change in embryos and larvae, suggesting that DLC contaminated sediment probably did not occur much beyond it. P1 extract resulted in concentration dependent increases in mortality and pericardial edema. Its highest concentration caused up-regulation of P-450 (CYP)-1A1(CYP1A) mRNA expression at 72 h post fertilization (hpf), an increase in its expression in gill arches as observed by whole mount in situ hybridization, and an increased signal in the Tg(cyp1a: mCherry) transgenic line. The pattern and magnitude of response was very similar to that of TCDD and supported the presence of DLCs in these sediment samples. Follow-up chemical analysis confirmed this presence and identified H7CDF, O8CDF and O8CDD as the main components in P1 extract. This study validates the use of biological assays as a rapid, sensitive, and cost-effective method to evaluate DLCs and their effects in sediment samples. Additionally, it provides support for the conclusion that DLCs have limited remobilization capacity in marine sediments.
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