期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 172, 期 -, 页码 1-10出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2019.01.044
关键词
Benzo[a]pyrene; Biodegradation; Proteomics; Ion; Enzyme
资金
- National Key Research and Development Program of China [2018YFC1803304, 2017YFD0801300]
- Science and Technology Project of Guangdong Province [2016B02024007]
- Foundation of Technology Research Center for Petrochemical Resources Clean Utilization of Guangdong Province [201516B10]
- Fundamental Research Funds for the Central Universities [21617453]
Benzo[a]pyrene (BaP) is a model compound of polycyclic aromatic hydrocarbons. The relationship between its toxicity and some target biomolecules has been investigated. To reveal the interactions of BaP biodegradation and metabolic network, BaP intermediates, proteome, carbon metabolism and ion transport were analyzed. The results show that 76% BaP was degraded by Brevibacillus brevis within 7 d through the cleavage of aromatic rings with the production of 1-naphthol and 2-naphthol. During this process, the expression of xylose isomerase was induced for xylose metabolism, whereas, alpha-cyclodextrin could no longer be metabolized. Lactic acid, acetic acid and oxalic acid at 0.1-1.2 mg dm(-3) were released stemming from their enhanced biosynthesis in the pathways of pyruvate metabolism and citrate cycle, while 5-7 mg dm(-3) of PO43- were transported for energy metabolism. The relative abundance of 43 proteins was significantly increased for pyruvate metabolism, citrate cycle, amino acid metabolism, purine metabolism, ribosome metabolism and protein synthesis.
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